The chromatographic separation of uroporphyrin I and III octamethyl esters.
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منابع مشابه
Chromatographic separation of uroporphyrins I and III and the nature of Waldenström porphyrin.
Patients with porphyria usually discharge large amounts of uroporphyrins, mainly as a mixture of the isomers I and III, as well as other porphyrins. Knowledge concerning their quantitative distribution in the disease has long been sought. The chromatographic separation of methyl esters of uroporphyrin I and “Waldenstrom porphyrin” (1, 2), then believed to be uroporphyrin III, was described by F...
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The novel heme prosthetic group of Escherichia colt’ NADPH-sulfite reductase (EC 1.8.1.2) has been identified as an iron tetrahydroporphyrin of the isobacteriochlorin type (adjacent pyrrole rings reduced) with eight carboxylic acid side chains. The number of carboxyl groups was established by the following experiments. Esterification with 14CH30H resulted in incorporation of 7.4 moles of 14C pe...
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Hepta-, hexa-, and pentacarboxylic porphyrins have been detected in porphyric as well as normal urine samples. From the former they have been isolated and converted into pure methyl esters (1). For a better understanding of the chemistry of these porphyrins, pure uroporphyrin I and uroporphyrin III were separately subjected to decarboxylation under controlled conditions. The decarboxylation pro...
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5. Specimens from Prof. C. J. Watson's laboratory have been examined. The uroporphyrin methyl ester I had m.p. 292-293° and behaved as uroporphyrin I containing a trace of uroporphyrin III on paper chromatography. The 2080 ester behaved chromatographically as a hexacarboxylic porphyrin. We wish to thank Dr Falk and Miss Benson for performing the chromatographic examinations by their method, and...
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5-Aminolevulinic acid is incubated with a crude enzyme extract from Rhodopseudomonas spheroides, mutant R 26. The formed porphyrins (main product: uroporphyrin III) are isolated. Incorporation o f iron, ring-splitting by coupled oxydation and subsequent iron removal leads to a mixture o f pigments, from which urobiliverdin, a new bile pigment with eight carboxylic acid side chains, is isolated....
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عنوان ژورنال:
- Analytical biochemistry
دوره 95 2 شماره
صفحات -
تاریخ انتشار 1979